Vitamin C acts indirectly to modulate isotype switching in mouse B cells / 대한해부학회지
Anatomy & Cell Biology
;
: 25-35, 2010.
Article
in English
| WPRIM
| ID: wpr-43659
ABSTRACT
Vitamin C, one of essential micronutrients, has been reported to modulate the humoral immune responses in some mammals. We investigated whether vitamin C might modulate this response in mice by directly affecting B cells. Splenic B cells were isolated and activated by CD40- and B cell receptor-ligation in vitro. The cells were cultured with a pretreatment of vitamin C from 0 to 1 mM of concentrations. Vitamin C slightly increased apoptosis of B cells dose-dependently and behaved as an antioxidant. We found that in vivo administration of vitamin C by intraperitoneal injection affected isotype switching as previously reported the titer of antigen-specific IgG1 antibody was decreased, while that of IgG2a was unaffected. Somewhat different from those observed in vivo, in vitro exposure to vitamin C slightly decreased isotype switching to IgG1 and increased isotype switching to IgG2a. Pretreatment with vitamin C in the safe range did not affect either proliferation of cultured B cells or the expression of CD80 and CD86 in those cells. Taken together, in vivo results suggest that vitamin C acts to modulate isotype switching in the mouse. However, because of our in vitro results, we suggest that the modulation exerted by vitamin C in vivo is by indirectly affecting B cells, perhaps by directly influencing other immune cells such as dendritic cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ascorbic Acid
/
Vitamins
/
Dendritic Cells
/
Immunoglobulin G
/
B-Lymphocytes
/
Reactive Oxygen Species
/
Apoptosis
/
Immunoglobulin Class Switching
/
Micronutrients
/
Immunity, Humoral
Limits:
Animals
Language:
English
Journal:
Anatomy & Cell Biology
Year:
2010
Type:
Article
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