Sequencing of Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium and application for PCR to differentiate them
Journal of Preventive Medicine
;
: 36-41, 2005.
Article
in Vietnamese
| WPRIM
| ID: wpr-4366
ABSTRACT
All Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium code for phase 1 of H antigen (H1) (d, a, b, c and i antigen respectively). The genes were sequenced on Sanger's principle by automatic sequenser (ABI, 3100 Avant, Genetic Analyser). The primer pairs for the Salmonella species were designed on the basis of the collected sequences. The results showed that PCR with these primers can clearly differentiate the five Salmonella strains, especially between S. paratyphi B and S. typhimurium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Salmonella
/
Polymerase Chain Reaction
Language:
Vietnamese
Journal:
Journal of Preventive Medicine
Year:
2005
Type:
Article
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