Determination of orientin and vitexin in Jinlianhua Pills by HPLC / 中国基层医药
Chinese Journal of Primary Medicine and Pharmacy
;
(12): 2914-2915, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-436753
ABSTRACT
Objective To develop a HPLC method for quantitative determination of orientin and vitexin in Jinlianhua Pills and to provide valuable data for quality control of Jinlianhua Pills.Methods The separation was performed on a Shimadzu VP-ODS column (250 x 4.6mm,5μm)at 35℃ using a mobile phase of 0.5% acetic acid solution-acetonitrile(8812).The detection wavelength was 340nm.Results The calibration curve of orientin showed a good linearly within test ranges of 25.0 ~ 75.0μg/ml.The average recovery was 99.9% with RSD 1.2%.The calibration curve of vitexin showed a good linearly within test ranges of 8.0 ~ 24.0μg/ml.The average recovery was 100.5% with RSD 1.3%.Conclusion The developed method is simple,accurate and with good repeatability.It is suitable for quality evaluation of Jinlianhua Pills.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Primary Medicine and Pharmacy
Year:
2013
Type:
Article
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