Mechanism of 2Gy60Coγ-ray-induced apoptosis in the regulatory T cells of mice / 中华放射医学与防护杂志

ABSTRACT

Objective To analyze the influence of ionizing radiation on the viability of the regulatory T cells and the relationship between the expression of CD39 and apoptosis related factors in mice Treg cells.Methods Mice were administered to whole body irradiation with 2 Gy of γ-rays,then lymphocytes were separated from thymus and spleen.The percentages of CD4 + T and CD4 + FOXP3 +CD25 + Treg lymphocytes,apoptosis related genes of Bax,Bcl-2 and CD39 were analyzed with FACS.Results Treg cells had a higher survival fraction than CD4 + FOXP3-Tcon cells after irradiation,and the time-responses of Treg cells in thymus and spleen were different.The apoptosis rate of Treg cells increased at 4 d after irradiation(t =-3.6,-7.4,P ＜0.05).The Bax/Bcl-2 ratio and the CD39 expression were increased in thymus Treg cells (t =-3.4,-6.6,P ＜ 0.05),but decreased in spleen Treg cells (t =2.4,2.6,P ＜0.05).Conclusions Thechanges of apoptosis and Bax/Bcl-2 are in consistent with the expression of CD39 in the irradiated Treg cells.