Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 5995-6000, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-437463
ABSTRACT
BACKGROUND:
Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material.OBJECTIVE:
To determine the potential toxic effects of ostrich corneal stromal scaffold on cel s.METHODS:
Cel culture methods were used to culture L-929 cel s in the extracts of ostrich acel ular corneal stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cel s after cultured for 1, 2 and 3 days. RESULTS ANDCONCLUSION:
After the cel s were cultured in the extracts of ostrich acel ular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cel s was graded as level 1. The cytotoxicity test was conducted according to the“National Standard of the People's Republic of China GB/T16886.5-2003”. After cultured in the extracts of ostrich acel ular corneal stroma, a smal number of cel s were round in shape and loosely adherent without intracytoplasmic granules, and cel lysis could be observed occasional y. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that the ostrich acel ular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2013
Type:
Article
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