Research progress on the detection methods of microRNA / 中华检验医学杂志

ABSTRACT

microRNA (miRNA) is a class of endogenous non-coding small RNAs,they play an important role in post-transcriptional regulation of gene expression through combining the target mRNA that the target protein would not synthesis.The present studies have found that miRNA is involved in many kinds of physiological processes,as well as the pathological processes.The abnormal expression of miRNA in many diseases can be used in diagnosis,prognosis and treatment monitoring.But all of these studies depend on an ideal detection method of miRNA.A lot of detection methods of miRNA expression developed from qualitative analysis to quantitative analysis step by step and from single miRNA detection to high throughput screening in recent years.Various detection methods are improved constantly,the specificity and sensitivity has been improved at the same time,detection procedure become simple and practicable,detection time shorten considerably and cost also reduced ceaselessly,which make the application of miRNA in clinical possible.This review highlights the latest research progress of the common detection methods of miRNA.