Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 645-656, 2008.
Article
in Korean
| WPRIM
| ID: wpr-43838
ABSTRACT
PURPOSE:
To evaulate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). MATERIALS ANDMETHODS:
1.2x10(-1)%, 1.2x10(-2)% and 1.2x10(-3)% CHX and 3x10(-3)%, 3x10(-4)% and 3x10(-5)% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.RESULTS:
The result were as follows 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2x10(-3) % CHX increased mRNA expressions of hPDLF as application time increased. 3. H2O2 lower than 3x10(-3) % increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2x10(-1) % CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2x10(-2) % CHX (with or without H2O2) for 30 minutes resulted in no gene expression.CONCLUSION:
Because low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Ligament
/
RNA, Messenger
/
Chlorhexidine
/
Fibronectins
/
Tissue Inhibitor of Metalloproteinase-1
/
Matrix Metalloproteinase 1
/
Collagen Type I
/
Fibroblasts
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2008
Type:
Article
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