Quality Standard for Rupikang Capsules / 中国中医药信息杂志

ABSTRACT

Objective To establish the quality standard for Rupikang Capsules. Methods Lumbricus were identified by TLC. Tanshinone IIA was determined by HPLC. HPLC analysis was carried out on Hitachi L-2000 C18 column (250 mm×4.6 mm, 5 μm) and the mixture of methanol-water (75∶25) was used as the mobile phase, the flow rate was 1.0 mL/min and the detection wavelength was at 270 nm. Results TLC spots developed were fairly clear and the blank test showed no interference. The linear range was 0.16-0.4 μg for tanshinone ⅡA, r=0.999 9. The average recovery was 99.1%, RSD=2.20%. Conclusion The methods is easy to operate, with good specificity and reproducibility. It can be used for the quality control of Rupikang Capsules.