Effect of Shenlongtang on Learning and Memory Ability and Expression of Bcl-2 and Bax in Hippocampus of Cerebral Ischaemia Rats / 中国中医药信息杂志

ABSTRACT

Objective To investigate the effect of Shenlongtang decoction on learning and memory ability and expression of Bcl-2 and Bax on rats hippocampus with cerebral ischemia model. Methods The cerebral ischemia animal model was established by two-vessel occlusion. Fifty rats were randomly divided into control group, model group, pisacetam group, Shenlongtang groups (low and high dosage). After modeling, rats were administrated with corresponding drugs for 28 days, the learning and memory ability was tested with Morris water maze, the number of positive cells and the integral optical density of the immunostaining on Bcl-2 and Bax protein expression in the hippocampus was detected. Results With Morris water maze test, the latency time increased significantly and times of searching submerged platform decreased significantly in the model group, the difference was significant compared with control group (P<0.01). Compared with model group, the latency time of Shenlongtang high dosage group decreased significantly and times of searching submerged platform increased significantly (P<0.05). Compared with model group, the expression of positive cells number of Bax protein expression in the hippocampus area was significantly increased (P<0.05), the Bcl-2 protein expression in the hippocampus region was significantly decreased (P<0.01). Conclusion Shenlongtang could significantly improve the learning and memory ability of cerebral ischemia rats, the mechanism may be related to regulating the expression of Bcl-2 and Bax.