Human Cytomegalovirus Induces Intercellular Adhesion Molecule-1 Expression in a Monocytic Cell Line, THP-1
Journal of Bacteriology and Virology
;
: 39-46, 2008.
Article
in Korean
| WPRIM
| ID: wpr-43953
ABSTRACT
It has been reported that inflammatory diseases such as pneumonitis, retinitis, and hepatitis are associated with human cytomegalovirus (HCMV). Intercellular adhesion molecule (ICAM)-1 is an important inflammatory mediator, helping monocytes adhere to endothelial cells when tissues are infected by pathogen including the HCMV. However, little is known about the mechanism of ICAM-1 stimulation by the HCMV infection in monocytes. In this study, a monocytic cell line THP-1 was used to understand ICAM-1 expression by the HCMV infection. Flow cytometric analyses demonstrated that ICAM-1 was stimulated by the HCMV in THP-1 cells with maximum at 24 hours post infection. The stimulated ICAM-1 expression was dependent on the amount of input virus. In order to understand the mechanism of ICAM-1 stimulation during the HCMV infection, cells were treated with specific inhibitors of key elements in inflammation NF-kappaB inhibitor PDTC, cyclooxygenase 2 inhibitor NS398, and MEK inhibitor PD98059. Flow cytometric analyses revealed that ICAM-1 expression was decreased when treated with PDTC, but not with NS398 or PD98059. Thus, it is suggested that HCMV-induced ICAM-1 expression in THP-1 cells is associates with NF-kappaB.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Retinitis
/
Sulfonamides
/
Thiocarbamates
/
Viruses
/
Flavonoids
/
Proline
/
Monocytes
/
Cell Line
/
NF-kappa B
Limits:
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2008
Type:
Article
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