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Expression of Sme Efflux Pumps and Multilocus Sequence Typing in Clinical Isolates of Stenotrophomonas maltophilia
Annals of Laboratory Medicine ; : 38-43, 2012.
Article in English | WPRIM | ID: wpr-43988
ABSTRACT

BACKGROUND:

Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen, which causes infections that are often difficult to manage because of the inherent resistance of the pathogen to a variety of antimicrobial agents. In this study, we analyzed the expressions of smeABC and smeDEF and their correlation with antimicrobial susceptibility. We also evaluated the genetic relatedness and epidemiological links among 33 isolates of S. maltophilia.

METHODS:

In total, 33 S. maltophilia strains were isolated from patients in a tertiary hospital in Daejeon. Minimum inhibitory concentrations (MICs) of 11 antimicrobial agents were determined by using agar dilution method and E-test (BioMerieux, France). Real-time PCR analysis was performed to evaluate the expression of the Sme efflux systems in the S. maltophilia isolates. Additionally, an epidemiological investigation was performed using multilocus sequence typing (MLST) assays.

RESULTS:

The findings of susceptibility testing showed that the majority of the S. maltophilia isolates were resistant to beta-lactams and aminoglycosides. Twenty-one clinical isolates overexpressed smeABC and showed high resistance to ciprofloxacin. Moreover, a high degree of genetic diversity was observed among the S. maltophilia isolates; 3 sequence types (STs) and 23 allelic profiles were observed.

CONCLUSIONS:

The smeABC efflux pump was associated with multidrug resistance in clinical isolates of S. maltophilia. In particular, smeABC efflux pumps appear to perform an important role in ciprofloxacin resistance of S. maltophilia. The MLST scheme for S. maltophilia represents a discriminatory typing method with stable markers and is appropriate for studying population structures.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacterial Proteins / Ciprofloxacin / Microbial Sensitivity Tests / Gene Expression Regulation, Bacterial / Bacterial Typing Techniques / Gram-Negative Bacterial Infections / Stenotrophomonas maltophilia / Drug Resistance, Multiple, Bacterial / Alleles / Multilocus Sequence Typing Limits: Humans Language: English Journal: Annals of Laboratory Medicine Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacterial Proteins / Ciprofloxacin / Microbial Sensitivity Tests / Gene Expression Regulation, Bacterial / Bacterial Typing Techniques / Gram-Negative Bacterial Infections / Stenotrophomonas maltophilia / Drug Resistance, Multiple, Bacterial / Alleles / Multilocus Sequence Typing Limits: Humans Language: English Journal: Annals of Laboratory Medicine Year: 2012 Type: Article