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The experimental research between magnetic resonance spectroscopy and changes of behavior for neural stem cell transplantation in APP/PS1 transgenic mice / 中华行为医学与脑科学杂志
Chinese Journal of Behavioral Medicine and Brain Science ; (12): 965-968, 2013.
Article in Chinese | WPRIM | ID: wpr-440277
ABSTRACT
Objective To explore the effect of neural stem cell(NSCs) transplantation on proton magnetic resonance spectroscopy (1H-MRS) and the behavior in APP/PS1 double transgenic AD mice.Methods NSCs from C57BL/6 mice were cultured and amplified.APP/PS1 double transgenic AD mice (n=30) aged 12 months were used as the study group,and mild-type mice (n=15) were used as the control group(group C).Animals in the study group were randomly divided into two subgroupsone receiving NSCs (group A) and the other receiving PBS transplantation (group B) in bilateral hippocampal CA1 of the AD model mice.Animals in the group C were not treated.1 H-MRS and Morris water maze (MWM) were performed before transplantation and 4 weeks after transplantation,and compared with the histopathological results.Results 1H-MRS showed that there was no significant change in NAA/Cr(1.01±0.08 and 1.03±0.05) and mI/Cr (0.69±0.05 and 0.71±0.06) ratios between group A and group B before transplantation (P> 0.05),but the changes were significant compared with the group C (NAA/ Cr1.21±0.05; mI/Cr0.58±0.06) (P<0.05).Four weeks after transplantation,NAA/ Cr ratio(1.18± 0.09) was increased and mI/Cr ratio (0.53±0.04) was decreased in group A.The difference was significant compared with the group B at the same time points (P<0.05).MWM showed the escape latency in group A was significantly shorter than that in group B after transplantation (P<0.05).In addition,group A also showed an exclusive preference for the target quadrant,and spent more time ((35.21±5.44) s) in the 3rd quadrant compared with group B (P<0.05).For number of platform crossings,similar results were also shown (5.75± 3.23).Nissl's staining showed that the number of neurons in the hippocampal area increased more significantly in group A than those in group B(P<0.05).Conclusion NSCs transplantation can improve spatial learning and memory via neurons regeneration in APP/PS1 double transgenic AD mice,and 1H-MRS is able to display intracranial metabolite changes after NSCs transplantation.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Behavioral Medicine and Brain Science Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Behavioral Medicine and Brain Science Year: 2013 Type: Article