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Lentivirus PARG-shRNA transfection decreases matrix adhesion,migration and invasion potencies of colon carcinoma lovo cells / 基础医学与临床
Basic & Clinical Medicine ; (12): 237-241, 2010.
Article in Chinese | WPRIM | ID: wpr-440645
ABSTRACT
Objective To explore the effects of poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion,migration and invasion.Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected.Western blot was used to test the expression of PARG,poly(ADP-ribose) polymerase(PARP) and NF-κB.The lovo cell matrix adhesion,migration and invasion potencies were observed by cell matrix adhesion,migration and invasion assay.Results The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (P<0.05).PARG gene silencing of lovo cells decreased the cell matrix adhesion,migration and invasion potencies.The inhibitory rates of lovo cell matrix adhesion,migration and invasion were 25.22%,38.71%,35.29% respectively.Conclusion PARG gene silencing of lovo cells could inhibite cell matrix adhesion,migration and invasion potencies,which may relate to that PARG gene silencing and decreasing the activity of PARP and NF-κB.PARG probably plays an important role in the infiltration and metastasis of tumor.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Basic & Clinical Medicine Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Basic & Clinical Medicine Year: 2010 Type: Article