Technology progress in the in vitro construction and culture of tissue-engineered bone / 中国组织工程研究

ABSTRACT

BACKGROUND:The in vitro construction, maturation and differentiation of cellscaffold complexes into tissue-engineered bone is the necessary process of bone tissue engineering construction, but there are no uniform methods and standards. OBJECTIVE:To summarize the basic method and technology to build bone tissue engineering at present and to discuss the related development. METHODS:A compute-based online search was conducted on the PubMed database and CNKI database for the articles related to the in vitro construction and culture of bone tissue engineering bone from January 1997 to January 2013 with the key words of“bone tissue engineering, cellbiological scaffold, cellinoculation, seeding density, culture in vitro, bioreactor”in English and Chinese. Final y, 44 articles were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:As the carrier of bone tissue engineering seed cells, the primary prerequisite of biological scaffold is sterile, because the sterile biological scaffold can be able to survive. Sterilization of biological scaffolds includes ultraviolet sterilization, 60Coγ-ray sterilization, soaking in ethanol with the volume fraction of 75%, autoclave method, and ethylene oxide sterilization. 60Coγ-ray sterilization is the common method in the biological scaffold sterilization. The inoculation density of seed cells is the key factors that influence the adhesion growth and proliferation of seed cells on the scaffolds. The adhesion between cells and scaffold materials wil be affected by the affinity of scaffolds, celladhesion and gravity, and other factors. The method for the inoculation of bone tissue engineering seed cells includes static inoculation and dynamic inoculation. Each construction method has its advantages and disadvantages. Overcomeing these disadvantages, forming a uniform construction method and ful y clinical application are the direction of future development.