The Effects of Autologous Skeletal Myoblasts Transplantation on Hurted Vocal Cord of Paralysis / 听力学及言语疾病杂志

ABSTRACT

Objective The goal of the present experimental study is to investigate effects on the injection of a-dult rabbit autologous skeletal myoblasts into the thyroarytenoid(TA)muscle after recurrent laryngeal nerve (RLN) damage and traumatic vocal cords .Methods 20 adult New Zealand white rabbits were used for the establishment of an animal model simulating the cut -off of the left recurrent laryngeal nerve and the left vocal cord trauma scar for-mation after traumatic damage .Then from the rabbits ,the sternocleidomastoid muscle cells were cultured ,separa-ted and expanded .After 12 weeks ,the 20 animals were randomly divided into group A ,group B with 10 in each group .Group A(myoblast injection group) in the left vocal cord thyroarytenoid muscle injection of myoblasts 0 .3 ml ,the right vocal cord without any treatment served as normal controls ;Group B(saline injection control group) in the left vocal cord thyroarytenoid muscle was injected with normal saline ,the right vocal cord without any treatment as normal controls ,respectively ,were observed after 12 weeks of bilateral thyroarytenoid muscle fiber diameters and vocal cord volumes .Results After 12 weeks ,Group A's left thyroarytenoid muscle injection of bilateral thyroaryten-oid muscle fiber diameter and the vocal volume size was not significantly different (P>0 .05) ,thyroarytenoid muscle fiber diameter and the vocal cord volume on Group B's injected side was significantly smaller than Group A (P<0 .05) .Conclusion The thyroarytenoid muscle injection of autologous myoblasts can effectively treat recurrent la-ryngeal nerve (RLN) injury and traumatic vocal cords .