Association Study between polymorphism of IL1β-31T/C,-511C/T and chronic obstructive pulmonary disease / 中国综合临床

ABSTRACT

Objective To investigate the relationship between polymorphism of interleukin-1β(IL-1β)-31,-511C/T and chronic obstructive pulmonary disease.Methods Two hundred and sixty patients with chronic obstructive pulmonary disease (COPD) were selected as our subjects who were hospitalized in General Hospital of Kailuan from January 2009 to June 2012.At same time,the 260 healthy controls were recruited in medical examine center.The data was collected by the physical examination and a unified questionnaire.Enzyme-linked immunosorbent assay (ELISA) was used to measure the level of IL-1β in serum.Polymerize chain reaction-Restriction fragment length polymorphism (PCR-RLFP) was used to detect the genotypes of IL-1β (-31,-511).Multivariate logistic regression was used to analyze the relations between risk factors with susceptibility of COPD.Results The level of serum IL-1β was (3.92 ± 0.42) μg/L in COPD group,higher than that in control group ((2.69 ± 0.11) μg/L,t =12.889,P ＜ 0.001).The frequency of genotype of IL-1β-31 site in COPD group were 20.8％ (54/260) for TT,58.1％ (151/260) for CT and 21.2％ (55/260) for CC respectively.Meanwhile IL-1β-31 genotype rate were 19.2％ (50/260),58.8％ (153/260) and 21.9％ (57/260) respectively in control group and no significant difference was found between two groups (x2 =0.203,P =0.904).The frequency of genotype of IL-1β-511 site were 20.0％ (52/260) for CC,63.1％ (164/260) for CT and 16.9％ (44/260) for TT in COPD group.The three genotype rate in control group were 21.9％ (57/260),60.8％ (158/260) and 17.3％ (45/260) respectively,and no significant difference was found between two groups (x2 =0.352,P =0.838).Moreover there was also no significant difference in terms of gender(P ＞ 0.05)Conclusion The concentration of IL-1β in serum in COPD group was higher than in control group.The polymorphism of-31 and-511 were proved non association with COPD.