X-linked inhibitor of apoptotic protein modified adipose-derived stem cells transplantation promotes the regeneration of myocardium and capillaries after myocardial infarction / 中华老年医学杂志

ABSTRACT

Objective To investigate the mechanism of transplantation of autologous adiposederived stem cells (ADSCs) and X-linked inhibitor of apoptosis protein (XIAP) modified ADSCs for the treatment of myocardial infarction (MI) by the histological detection method.Methods Totally 27 male SD rats (aged 10 weeks,weighted 200-300 g) were randomly divided into the control group,ADSCs group and XIAP ADSCs group (n=9 each).ADSCs were isolated from inguinal adipose tissue in rats and were cultivated in Dulbecco' s modified Eagle' s medium.X-linked inhibitor of apoptotic protein expression plasmid was electrotransfected into ADSCs.Myocardial infarction model was conducted by ligating the left anterior descending artery.The control group was not given any operation.Rats in different groups received direct epicardial injections of normal saline,or ADSCs cell suspension or XIAP modified ADSCs cell suspensions respectively at five sites in the central and border zones of myocardial infarction.Results Compared with control group,the infarct areas were decreased in ADSCs and XIAP ADSCs groups,and some myocardial cells and blood capillaries could be found in the myocardial infarction zone.The capillary number was much more in XIAP-ADSCs group than in ADSCs and control groups [(18.65±1.12) /mm2 vs.(8.68±0.90) /mm2,(18.65±1.12) /mm2 vs.(7.64 ± 0.60)/mm2,t=-22.49,29.91,both P＜0.05].Conclusions Autologus ADSCs and XIAP-ADSCs transplantation may reduce infarction area and inhibit left ventricular remodeling by promoting the regeneration of myocardial cells and blood capillaries,which restores the cardiac function.