COX-2 mRNA expression at different stages of osteoarthritis synoviocytes / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1003-1008, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-444732
ABSTRACT
BACKGROUND:
COX-2 gene actual y exists in the joint fibroblast-like synoviocytes, it affects osteoarthritis occurrence and development. Understanding the differences of COX-2 gene expression levels at different stages of osteoarthritis synoviocytes has important theoretical significance for the occurrence and development of osteoarthritis, as wel as the role of synoviocytes in this process.OBJECTIVE:
To analyze the difference of COX-2 mRNA at different stages of osteoarthritis fibroblast-like synoviocytes.METHODS:
Synovial membrane from 44 osteoarthritis patients and 12 normal cases were selected. Primary cells were cultured to passage 4 fibroblast-like synoviocytes for the use in the experiment. COX-2 mRNA expression in osteoarthritis fibroblast-like synoviocytes and normal fibroblast-like synoviocytes was detected using real-time fluorescence quantitative RT-PCR. The relative quantitative analysis was performed using 2-ΔΔCt method. RESULTS ANDCONCLUSION:
Expression of COX-2 mRNA in osteoarthritis fibroblast-like synoviocytes was significantly higher than that in normal fibroblast-like synoviocytes (P0.05). COX-2 mRNA might be important biological marker for the inflammation in osteoarthritis, and mainly plays a role in early osteoarthritis stage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2014
Type:
Article
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