Large vascularized tissue-engineered bone for repair of bone defects / 中国组织工程研究

ABSTRACT

BACKGROUND:Currently, there are many problems existing in the construction of large tissue-engineered bone, and the most important is ischemic necrosis. Vascularization is critical for the biological function of large tissue-engineered bone. OBJECTIVE:To prepare large pieces of vascularized tissue-engineered bone for orthotopic transplantation in repairing large femoral defects of rabbits, and to explore tissue regeneration methods and characteristics. METHODS:Rabbit femoral structure was firstly imitated to construct a vascularized prefabricated model, based on which, we constructed large pieces of vascularized tissue-engineered bone. A total of 18 New Zealand white rabbit 18, aged 8 weeks, were divided into experimental group and control group. The experimental group was subject to orthotopic transplantation of large vascularized tissue-engineered bone, while the control group was treated with orthotopic transplantation of large tissue-engineered bone without vascularization processing. After 2, 4, 8 weeks, gross observation, X-ray films, and tissue section were compared between the two groups. RESULTS AND CONCLUSION:Gross observation and imaging observation showed that the osteogenesis in the experimental group at 2, 4, 8 weeks were better than that in the control group. The ratio of new bone tissue to total area of bone graft was significantly higher in the experimental group than the control group at 2, 4, 8 weeks postoperatively (P<0.01). These findings indicate that large vascularized tissue-engineered bone is preferred to the repair of large bone defects.