Preparation and biocompatibility of gamma-aminopropyl triethoxysilane/polypyrrole/polyester monofilament / 中国组织工程研究

ABSTRACT

BACKGROUND:Now most of the researches on transplant hair are based on the patients with enough hair, but there is no apparent corrective effect for large area of alopecia. Implanted artificial hairs can solve this problem. OBJECTIVE:To make and evaluate the γ-aminopropyl triethoxysilane/polypyrrole/polyester (KH-550/PPy/PET) composite monofilament as implanted artificial hairs, and to carry out cytotoxicity tests using NCTC clone 929 cels with composite monofilament. METHODS:KH-550/PPy/PET composite monofilament was prepared in a series of steps, including pre-spotting, alkali treatment, silane coupling agent treatment and polypyrrole coating. The viability of NCTC clone 929 cels were detected after 1, 2, 3, 5, 7 days of co-culture with composite monofilament by using cellcounting kit-8. RESULTS AND CONCLUSION:KH-550/PPy/PET composite monofilament had a smooth surface without crack. The PPy films did not come off accidentaly and had good wearability. After alkali treatment, PPy quality on the surface of monofilament was significantly heavier than before. Using silane coupling agent (KH-550) could effectively enhance biocompatibility and binding force of polyester monofilaments. After co-cultured with composite monofilaments, the viability of NCTC clone 929 cels was 100%, 80.37%, 73.26%, 81.96%, 77.50% at days 1, 2, 3, 5, 7 respectively. The level of cytotoxicity was grade 1. The results show that KH-550 can effectively enhance the binding force between PPy and PET monofilament, and the prepared KH-550/PPy/PET composite monofilament has good biocompatibility and no acute toxicity.