Insulin-like growth factor-1/chitosan/collagen composite scaffold and the proliferation of human periodontal ligament cells / 中国组织工程研究

ABSTRACT

BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix. OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels. METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28. RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.