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The effects of different titanium alloy surface topograph on the biological character of fibroblasts / 实用口腔医学杂志
Journal of Practical Stomatology ; (6): 151-155, 2014.
Article in Chinese | WPRIM | ID: wpr-444900
ABSTRACT

Objective:

To compare the effect of three different titanium alloy surface topographies on the biological character fibro-blasts.

Methods:

Three different titanium alloy Ti6Al4V surface topographies were created by machining(M),direct current electro-chemical etching(DC)and alternating current electrochemical etching(AC)respectively,and standard sized samples were prepared. The surface topographies were observed field emission scanning electron microscopy(FE-SEM).Mouse L929 fibroblasts were cultured on different surfaces.The attachment of cells was examined by acridine orange staining and observed under fluorescent microscope.The proliferation of cells was measured using MTT assay.

Results:

Tubles with the diamete of 5 -20 μm were observed on the surface of the sample of group DC,and more evenly distributed tubles on that of group AC.Micro-sulci were observed and no microtuble was found on the sample surface of group M.L929 cells were attached on the surface of all the samples in the 3 groups.More adhered and better strached cells were observed on AC treated surface.The proliferation of fibroblasts on AC and DC surfaces was superior to that on M treated surface and at the 3 -5 day the proliferation of fibroblasts on the AC surface was higher than that on DC treated surface.Con-clusionAC treated surface with the micro-submicro-nano hierarchical topography of titanium alloy has a superior biocompatibility and can promote the early attachment and proliferation of the fibroblasts.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2014 Type: Article