Effects of tobacco extract on the proliferation and HSP70 expression of human periodontal ligament cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 211-214, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-445215
ABSTRACT
Objective:
To study the effects of smokeless tobacco extract(ST)on the proliferation and the heat shock protein 70 (HSP70)expression of human periodontal ligament cells(hPDLCs).Methods:
hPDLCs were cultured in vitro and identified by im-munohistochemistry(IHC).The cells were stimulated with ST at 0.01 6 -50 g/L respectively for 24 h,the proliferation was examine by MTT assay,HSP70 expression was detected by immunehistochemical staining and Western Blot.Results:
ST inhibited the prolifer-ation and increased HSP70 expression in cytoplasm and nucleus at 0.4 -50 g/L dose dependantly.Conclusion:
ST may inhibit the proliferation and increase HSP70 expression of hPDLCs in a dose depandant manner.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2014
Type:
Article
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