Determination of Benzoic Acid and Cinnamic Acid in Suhexiang Pills by HPLC with Dual Wavelength De-tection / 中国药师
China Pharmacist
;
(12): 692-694, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-445906
ABSTRACT
Objective:
To establish an HPLC method with dual wavelength detection for the determination of benzoic acid and cin-namic acid in Suhexiang pills.Methods:
A Waters Sun Fire TM C18 column(150 mm × 4. 6 mm,5 μm) was adopted with 0. 1% ace-tic acid-methanol(60∶40) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 228nm for benzoic acid(0-18min) and 285nm for cinnamic acid (18. 1-35min). The injection volume was 10μl, and the column temperature was room temperature.Results:
The linear range of benzoic acid and cinnamic acid was 1. 0-50. 0 μg·ml-1(r=0. 999 7) and 0. 2-10. 0 μg· ml-1, respectively(r=0. 999 9). The average recovery was 96. 88%(RSD=1. 6%, n=9) and 99. 35%(RSD=1. 7%, n=9), re-spectively.Conclusion:
The method is accurate, fast and simple in the determination of benzoic acid and cinnamic acid in Suhexiang pills.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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