Effects of Artesunate on K562, K562/ADM Cell Apoptosis and NF-κB p65 Expression in Human Leukemia / 中国药师
China Pharmacist
;
(12): 729-731, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-446013
ABSTRACT
Objective:
To study the effects of artesunate on K562, K562 /ADM cell apoptosis and NF-κB p65 expression in hu-man leukemia.Methods:
The effect of artesunate with different concentrations on K562 and K562 /ADM cell apoptosis at various times was studied by a flow cytometer. The effect of artesunate with 15 μmol·L-1 on NF-κB P65 expression in K562 was explored u-sing a Western blot method.Results:
Artesunate showed little effect on K562 cell apoptosis, while exhibited notable effect on K562 /ADM cell apoptosis. K562 /ADM cells were more sensitive than K562 to artesunate at the concentration of 7. 5 μmol·L-1 and 15μmol·L-1(P<0. 01), and after 4-hour treatment with 15 μmol·L-1 artesunate, K562 /ADM cell apoptosis was much higher than K562(P<0. 01). Artesunate could reduce leukemia cell p65 expression at the concentration of 15 μmol·L-1.Conclusion:
Artesu-nate can induce the apoptosis of leukemia cells by down-regulating the expression of p65.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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