Effect of Adenovirus-p53 to Non-Small Cell Lung Cancer Cell Lines / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 1134-1146, 1998.
Article
in Korean
| WPRIM
| ID: wpr-44622
ABSTRACT
BACKGROUND:
The tumor suppressor gene p53 is one of the most frequently altered genes in human tumors, including those of the lung. There is now a compelling evidence that wild-type p53 can negatively influence cell growth by causing G1 arrest or by inducing apoptosis. The possibilities of using p53 for gene therapy are also gathering much interest. MATERIAL ANDMETHOD:
Our approach towards understanding p53 function would be to study the biological consequences of overexpression of wild-type p53 in normal and tumor cells by using adenovirus vectors capable of giving high levels of the p53 gene product in cells. We have used this vector containing wild-type p53 to infect tumor cells with different p53 status (null, mutant, or wild-type) to confirm that expression of p53 in null or mutant cell lines becomes possible by Adenovirus-p53 transduction, to examine the effects of high levels of p53 expression on the growth properties of tumor cells, to evaluate the role of apoptosis in p53-mediated biological effects, and to examine the effect of Adenovirus-p53 on the tumorigenicities of the lung cancer cell lines in vitro.RESULT:
The results of our study showed that cells expressing endogenous mutant p53 and those devoid of p53 expression altogether were significantly more sensitive to Adenovirus-p53-mediated cytotoxicity compared to tumor cells expressing endogenous wild-type p53 and that overexpression of wild-type p53 induced programmed cell death. Also we knew that Adenovirus-p53 significantly reduced tumor colony formation of human non-small cell lung cancer cell lines, and decreased the growth of preformed colonies in vitro.CONCLUSION:
These results suggest that adenovirus is an efficient vector for mediating transfer and expression of tumor suppressor genes in human non-small cell lung cancer cells and that the tumor cells null for p53 or expressing mutant p53 readily undergo apoptosis by Adenovirus-p53.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Therapy
/
Cell Line
/
Adenoviridae
/
Genes, p53
/
Genes, Tumor Suppressor
/
Negotiating
/
Cell Death
/
Apoptosis
/
Carcinoma, Non-Small-Cell Lung
/
Lung
Limits:
Humans
Language:
Korean
Journal:
The Korean Journal of Thoracic and Cardiovascular Surgery
Year:
1998
Type:
Article
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