Molecular Identification of the Traditional Chinese Medicine of the Deers Using COI Barcode Sequence / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
;
(12): 274-278, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-446433
ABSTRACT
Objective:
Using the COI Barcode to establish the standard method to identify the traditional Chinese medicine of deer products.Methods:
In this study, DNA extraction, PCR amplification, and sequencing of experimen-tal samples (deer antler, penis and testis, tendon, tail, bone, foetus) were successful. COI sequence database were constructed and commercial crude drugs of deer were investigated and analyzed.Results:
By using universal COI primer,PCR amplification is preferably. All the species could be identified based on COI sequences database of tra-ditional Chinese medicine of deer which contained 101 samples of 8 species. We have collected 40 commercial crude drugs in which 18 samples were identified as species described in Chinese pharmacopoeia.Conclusion:
DNA barcode technology based on COI sequence could be a standard approach to identify traditional Chinese medicine of deer products, and provide the basis for the identification of commercial deer medicine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
World Science and Technology-Modernization of Traditional Chinese Medicine
Year:
2014
Type:
Article
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