Correlation of expressions of TGF-β1, Smad7 and ki-67 mRNA and the clinicopathologic characteristics in gastric carcinoma / 实用医学杂志

ABSTRACT

Objective To investigate relationship between the expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA and the clinicopathologic characteristics in gastric carcinoma. Methods 60 cases with gastric carcinoma tissue and 20 cases with normal gastric tissue, which were surgically removed and certified by pathology, were collected. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA were detected by RT-PCR in normal and carcinomatous gastric tissue. Results The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in normal gastric tissue were lower than those in carcinomatous gastric tissue (P < 0.05). The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma which were well differentiated and moderately differentiated were significantly lower than those in poorly differentiated gastric carcinoma (P<0.05). The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma without lymph node metastasis were lower than those in gastric carcinoma with lymph node metastasis. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in Ⅰ+Ⅱ stage were lower those in Ⅲ+Ⅳ stage (P < 0.05). Linear correlation showed there were positive correlations among TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma. Conclusions The canonical TGF-β1 Smad7 and Ki-67 mRNA play a role in the carcinogenesis, development, differentiation of gastric carcinoma.