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Systematic establishment for directed differentiation of induced pluripotent stem cells into hematopoietic progenitor cellsin vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 3005-3011, 2014.
Article in Chinese | WPRIM | ID: wpr-446561
ABSTRACT

BACKGROUND:

There have been a large number of reports on establishing induced pluripotent stem celllines, but studies concerning large-scale in vitro induced differentiation of induced pluripotent stem cels into hematopoietic progenitor cels stil have a lack of in-depth discussion.

OBJECTIVE:

To develop methods to induce differentiation of induced pluripotent stem cels into hematopoietic progenitor cels in vitro.

METHODS:

Using the method of infection with lentivirus particles containing four transcriptionfactor genes, which are Oct4, Sox2, Nanog and Lin28, human skin fibroblasts are transduced into induced pluripotent stem cels. In the induced differentiation system, Y-27632, a kind of tyrosine kinase inhibitor-ROCK (p160-Rho-associated coiled-coil kinase), was added, which obviously suppressed apoptosis of cels. Based on conditioned medium with OP9 cels, a differentiation system of inducing induced pluripotent stem cels differentiating into hematopoietic progenitor cels was established. RESULTS AND

CONCLUSION:

(1) Apoptosis of induced pluripotent stem cels at the first three passages was very obvious, and the cels were difficult in a large-scale expansion. After Y-27632 was added, the apoptosis of embryonic stem cels was obviously inhibited. (2) During embryoid body differentiation, induced pluripotent stem cels cultured in OP9 conditional growth medium differentiated into hematopoietic progenitor celsin vitro that were positive for CD34.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2014 Type: Article