Cyclic stress promotes the proliferation of rabbit condyle chondrocytes / 中国组织工程研究

ABSTRACT

BACKGROUND:The condyle is one of important areas of lower mandible growth, and has growth remodeling capacity lifetime. The in vivo functional research fails to obtain satisfactory outcome due to the complexity of physiological environment, nondirectiveness of stimulating factor transduction, and difficulty to control experimental conditions. Further studies wil explore the effect of stress on condyle chondrocytes in vitro. OBJECTIVE:To observe the effect of cyclic stress on the proliferation of rabbit condyle chondrocytes cultured in vitro. METHODS:Rabbit condyle chondrocytes were separated and cultured in vitro. Passage 3 chondrocytes were subject to cyclic tensile stress (10%surface elongation, 6 cycles/min) for 1, 6, 12, 24 hours. While those cells without stress served as the control group. The cellcycle changes were detected with flow cytometry analysis, and the cellproliferation were analyzed by MTT assay. RESULTS AND CONCLUSION:The flow cytometry analysis showed that S-phase promoting factor exhibited significant differences at 6 and 12 hours compared with control group, and got the maximum value at 24 hours (P<0.01). MTT assay results showed that the cells proliferation at 6 and 12 hours exhibited significant differences compared with the control group, and got the maximum value at 24 hours (P<0.01). The cyclic stress can obviously promote the proliferation of condyle chondrocytes, and the stimulative effect can be sustained 24 hours.