α7nAChR is involved in anti-inflammation of physiological concentrations of glucocorticoids / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 706-710, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-446895
ABSTRACT
AIM:
To explore the role of α7 nicotinic acetylcholine receptor (α7nAChR) in anti-inflammation of glucocorticoids (GCs) at physiological concentrations .METHODS:
MTT assay was used to measure the viability of BV-2 cells, which were processed by hydrocortisone at different concentrations .On the basis of inflammatory model induced by LPS in BV-2 cells, experimental groups were divided as follows(1) control;(2) LPS;(3) GCs+LPS;(4) methyl-lycaconitine ( MLA)+GCs+LPS.The levels of TNF-αand IL-1βin the cell supernatants were detected by ELISA .RE-SULTSHydrocortisone at concentrations of 2 000 and 1 000 nmol/L decreased the cell viability to (76.9 ±5.5)% and (90.8 ±7.3)%, respectively, indicating the cellular injury by GCs at over-physiological doses.LPS significantly induced the releases of TNF-αand IL-1βin a time-and dose-dependent manner in BV-2 cells.Hydrocortisone at physiological con-centrations (500 and 250 nmol/L) reduced the releases of TNF-αand IL-1βin BV-2 cells stimulated by LPS, and MLA at concentration of 10 nmol/L antagonized the anti-inflammatory effect of GCs .CONCLUSION:
α7nAChR is involved in the anti-inflammatory effect of the physiological concentrations of GCs .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2014
Type:
Article
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