Biological axis of CXC chemokine ligand and expression of stem cell markers in human colorectal cancer tissues as well as their relationship with clinical and pathological characteristics / 中国组织工程研究

ABSTRACT

BACKGROUND:The biological axis of CXC chemokine ligand 12 (CXCL12)/CXC chemokine receptor 4 (CXCR4) plays an important role in the specific metastasis of tumors, whereas the mRNA expression of glycoprotein hormone receptor 5, a stem cellmarker, exerts an important effects on tumor proliferation, invasion and metastasis. OBJECTIVE:To investigate the expressions of CXCL12/CXCR4 and glycoprotein hormone receptor 5 in human colorectal cancer tissues as wel as their relationship with clinical and pathological characteristics. METHODS:Totaly 100 colorectal cancer patients admitted at Liaoning Cancer Hospital & Institute from January to June 2013 were enroled as experimental group, and another 100 healthy volunteers served as control group. Immunohistochemical SP method was used to detect the expression of CXCL12, CXCR4 and glycoprotein hormone receptor 5 mRNA in human colorectal cancer tissues. We analyzed the correlation of CXCL12, CXCR4 and glycoprotein hormone receptor 5 mRNA expression with colorectal cancer patients’ age, sex, tumor size and site, lymphatic metastasis and prognosis . RESULTS AND CONCLUSION:There was a higher expression of CXCR4 and glycoprotein hormone receptor 5 mRNA, but lower expression of CXCL12 mRNA in the human colorectal cancer tissues. The mRNA expression of CXCR4, CXCL12 and glycoprotein hormone receptor 5 showed no correlation with age, gender, tumor size and site of colorectal cancer patients, but was related to lymphatic metastasis. For colorectal cancer patients with lymphatic metastasis, the expression of CXCR4 and glycoprotein hormone receptor 5 mRNA was higher, but no change was found in the expression of CXCL12 mRNA. The expression of CXCR4 was increased with the degree of tumor malignancy as wel as glycoprotein hormone receptor 5 expressed on the surface of gastrointestinal cancer and brain tumor stem cels. These findings indicate that the growth and metastasis of human colorectal cancer tissues are promoted by increasing the expression of CXCR4 and glycoprotein hormone receptor 5 in human colorectal cancer tissues; the CXCL12/CXCR4 biological axis and glycoprotein hormone receptor 5 are expected to become a new target therapy for tumor diagnosis and treatment.