Human umbilical cord mesenchymal stem cells：isolation, identification and multi-directional differentiation / 中国组织工程研究

ABSTRACT

BACKGROUND:The umbilical cord mesenchymal stem cels can be obtained conveniently, noninvasively, without ethical restrictions, which are more original that stem cels from other sources and have little immunogenicity. Therefore, umbilical cord mesenchymal stem cels are a kind of ideal seed cels with promising application prospects. OBJECTIVE: To induce the differentiation of mesenchymal stem cels derived from human umbilical cord into adipocytes and osteoblasts. METHODS: The umbilical cord mesenchymal stem cels were isolated by tissue explants method. The morphology, proliferation and immunophenotype of the 3rd passage cels were analyzed, and then cels were induced to adipocytes and osteoblastsin vitro. The expressions of CD90, CD105, CD34 and CD15 were detected by flow cytometry. RESULTS AND CONCLUSION:The umbilical cord mesenchymal stem cels isolated by tissue explants method could be cultured and proliferatedin vitro. Flow cytometry analysis revealed that the cels were strongly positive for CD90 and CD105, but negative for CD34 and CD45. The umbilical cord mesenchymal stem cels could be induced to adipocytes and osteoblastsin vitro. These findings indicate that mesenchymal stem cels can be successfuly obtained from human umbilical cord by tissue explants method and have the potential of multi-directional differentiation.