Preparation and Determination of Encapsulation Efficiency and Content of Bererine Hydrochloride Lipo-somes / 中国药师
China Pharmacist
;
(12): 601-605, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-447315
ABSTRACT
Objective:
To prepare bererine hydrochloride ( BER) liposomes and establish an effective method for the determination of content and entrapment efficiency.Methods:
BER liposomes were prepared by ammonium sulfate gradient method. The encapsula-tion efficiency of BER liposomes was respectively determined by supercentrifugation method, microcolumn gel method and ultrafiltration method, and the content of every component in BER liposomes was detected by HPLC-ELSD.Results:
The results showed that super-centrifugation method could precisely separate the free drug from the liposomes. The optimum parameters of supercentrifugation method were the centrifugal speed of 60 000 r·min-1 , the centrifugal time of 1 h, the centrifugal temperature of 10℃ and the lipid concentra-tion of 6 mg·ml-1 .Conclusion:
The method is simple and sensitive with good separation efficiency. HPLC-ELSD can be used to de-termine the content of every component in BER liposomes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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