Simultaneous Determination of Eprosartan/Hydrochlorothiazide Tablets by HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the simultaneous determination of eprosartan/hydrochlorothiazide tablets. Methods:Isocratic separation was achieved on a Phenomenex C18 column(250 mm × 4. 6 mm, 5 μm) using the mobile phase com-posed of 0. 5% formic acid-acetonitrile(60∶40, pH 2. 80). The flow rate was 1. 0 ml·min-1, the detection wavelength was 272nm, the column temperature was 30℃ and the injection volume was 20μl. Results:The linearity between peak area and concentration was observed within the range of 60. 0-1 200. 0 mg·L-1(r=0. 999 9) for eprosartan and 1. 25-25. 00 mg·L-1(r=0. 999 9) for hydro-chlorothiazide. The mean recovery of eprosartan and hydrochlorothiazide was 100. 02%(RSD=0. 35%, n=9) and 97. 93%(RSD=1. 54%, n=9), respectively. Conclusion:The method is simple, sensitive and accurate, and can be applied in the determination of eprosartan/hydrochlorothiazide tablets.