A study on 6-gingerol attenuate vascular smooth muscle cells senescence through inhibition of mTOR pathway molecular / 重庆医学

ABSTRACT

Objective To investigate the effects of 6‐gingerol on the rat aortic vascular smooth muscle cells (VSMCs)senescence induced by angiotensin Ⅱ(Ang Ⅱ) .Methods Culturing rat VSMCs were stimulated with Ang Ⅱ with or without pretreating with 6‐gingerol .The senescent status of cells was verified by in situ staining for senescence‐associated β‐galactosidase(SA‐β‐gal) .The cell cycle distribution was analyzed by FACS .The protein expression of mTOR and phosphorylated P70‐S6K were detected by Western blot .Results When VSMCs were exposed to AngⅡ ,the senescence marker such as SA‐β‐gal staining and cell cycle arrest‐ing in G0/G1 phage were significantly increased .Furthermore ,the protein level of mTOR ,phosphorylated P70‐S6K were also en‐hanced significantly .However ,6‐gingerol was found to markedly inhibit the senescence‐like phenotype and related signal molecular induced by AngⅡ as mentioned above .Conclusion 6‐gingerol attenuate VSMCs senescence through inhibition of mTOR/P70S6K pathway molecular .