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Effect of Gambogic acid on proliferation and invasion in colon cancer LoVo cell line / 国际中医中药杂志
International Journal of Traditional Chinese Medicine ; (6): 220-222, 2014.
Article in Chinese | WPRIM | ID: wpr-447645
ABSTRACT
Objective To investigate the effects of gambogic acid on proliferation,invasion and protein expression of MMP-2 in LoVo human colon cancer cell line.Methods The CCK-8 assay was used to determine the anti-proliferation ability.Transwell chamber assay was used to study the invasion of LoVo colon cancer cells.Western Blot was applied to examine the protein expression of MMP-2.Results CCK-8 assay showed that after cells treated with 1,2,4 μmol/L GA for 24 h,48 h the inhibition rates were(0.16±0.11)%,(0.24±0.08)%,(0.58±0.01)%,(0.67±0.03)%,(0.79±0.01)% and (0.27±0.05)%,(0.69±0.09)%,(0.85±0.01)%,(0.87±0.01)%,(0.89±0.01)%,and there had statistically significant differences between control group (0 μmo/L) with experimental group (P<0.05).Gambogic acid can effectively inhibit LoVo cells invasion at a low-dose concentration,which the numbers of cells that digested the matrigel in control group (0 μmo/L)was 120.50± 8.69 and 69.83 ±4.75 in experimental group (1 μnol/L),P< 0.05).Western Blot revealed that Gambogic acid can down-regulate the expression of MMP-2,which the levels of down-regulating the expression of MMP-2 after treated with 1,2,4 umol/L GA were 48.67%,74.72%and 82.70% (P<0.05) Conclusion Gambogic acid can effectively inhibit the growth and invasion of the LoVo cells,which may be related with down-regulating the expression of MMP-2.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2014 Type: Article