Gene Polymorphism of Adiponectin in Han Nationality with Type 2 Diabetes Mellitus from Guangxi / 中国药师

ABSTRACT

Objective:To evaluate the relationship between single nucleotide polymorphism (SNP) +45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus (T2DM), obesity and insulin resistance (IR) in Han population in Guangxi. Methods:Polymer-ase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) was performed to identify the T-G polymorphism of adiponec-tin (exon 2) in 313 unrelated Han people from Guangxi including 95 normal glucose tolerance,103 impaired glucose tolerance and 115 type 2 diabetes mellitus. Results:In Han people of Guangxi, the SNP +45 GG genotype in adiponectin gene had significantly lower HDL-C levels than TT and TG genotypes in Han people(P<0. 05). The subjects with the GG genotype at position 45 had a signifi-cantly increased risk of IR (odds ratio of 2. 876[95%CI1. 141-7. 248]) compared with those with the TT genotype at position 45. Conclusion:In Han people of Guangxi, the SNP +45 TT genotype in adiponectin gene has significantly higher HDL-C levels than GG genotype. The subjects with the GG genotype at position 45 have a significantly increased risk of IR compared with those with the TT genotype, while obesity and T2DM are independent on SNP+45 of adiponectin gene.