Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
;
(6): 62-65, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-448291
ABSTRACT
Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis.Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed , respectively.The morphology of sperm from thawed epididy-mis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants .Results The sperms from thawed epididymis of the three groups were all well-preserved structural-ly.The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solu-tions, respectively, and there were significant differences between the three cryopreservation groups (P<0.05 for all). The number of pups from the DMSO , PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation , respectively.Conclusions All the three cryoprotectants DMSO , PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis.But PROH is the preference of these cryoprotectant solutions .
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Laboratorium Animalis Scientia Sinica
Year:
2014
Type:
Article
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