Bone marrow mesenchymal stem cells inhibit inflammatory response of microglial cells / 中国实验动物学报

ABSTRACT

Objective To investigate the inhibitory effect of bone marrow mesenchymal stem cells ( BMMSC) on the inflammatory response of microglial cells .Methods The samples were divided into four groups .Group Imicroglial (BV-2) cells were cultured in DMEM (high glucose).Group II BV-2 cells were cultured in DMEM containing lipopo-lysaccharide (LPS).Group IIIBV-2cells and BMMSCs were co-cultured in DMEM.Group IV BMMSCs were cultured in DMEM containing LPS .The growth state and ultrastructure of BV-2 cells were observed and the changes of TNF-αex-pression were detected .Results Different cell densities of BV-2 cells were observed under the optical microscope in an or-der from high level to low levelgroup I >group III >group II.The expressions of TNF-αweregroup Ⅱ >groupⅢ>group Ⅰ.Ultrastructural observation of BV-2 cells showed that there were a large number of swollen mitochondria and endoplasmic reticulum , some of them showed vacuolization .No BV-2 cells with multiple hucleoli were found in the group II indicating the absence of active cell growth .At the same time, cytolysis was observed only in the group II .The growth of BV-2 cells in the group III was better than that in the group II .Conclusions BMMSCs can inhibit inflammatory response of microglial cells, therefore, play a neuroprotective role.