Clinical significance of combined detection of serum pre-S1,pre-S2 antigen and HBeAg for diagnosis of HBV infection / 国际检验医学杂志

ABSTRACT

Objective To investigate the clinical significance of combned detection of serum pre-S1 ,pre-S2 antigen and hepatitis B virus(HBV) e antigen(HBeAg) for diagnosis of HBV infection .Methods Enzyme-linked immunosorbent assay(ELISA) was employed to detect the serum pre-S1 ,pre-S2 antigen and HBeAg of 450 patients with chronic hepatitis B and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect their HBV DNA .Results In patients with different HBV DNA levels ,serum pre-S1 ,pre-S2 antigen and HBeAg showed different expression ,the higher the load ,the higher the expression (P<0 .05) .Pre-S1 ,pre-S2 antigen and HBeAg were all correlated with HBV DNA (P<0 .05) and the descending order of rele-vance were HBeAg ,pre-S1 and pre-S2 antigen .Combied detection of the three indicators showed the highest correlation with HBV DNA .The positive proportion of HBV DNA of patients with the three indicators positive was markedly higher than those in pa-tients with single or two indicators positive (P<0 .05) .Conclusion Combind detection of serum pre-S1 ,pre-S2 antigen and HBeAg may reflect well on HBV replication .