Effects of 5-fluorouracil with 1-methyl tryptophan on myeloid-derived suppressor cells in gastric cancer bearing 615 mice / 中华普通外科杂志

ABSTRACT

Objective To investigate the effect of 1-MT in combination with 5-FU on the number of myeloid-derived supressor cells (MDSC) in gastric carcinoma beating mice.Methods By using the lipofectamine TM 2000,the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 (+) were transfected in a MFC cell line.Animal model of gastric cancer bearing 615 mice were established to give respectively normal saline (NS),1-MT,5-FU and 1-MT + 5-FU therapy.Flow cytometry and immunofluorescence were used to analyze MDSC expression in tumor tissue.Result (1) The expression of MDSC was detected in transplanted tumor.(2)The expression of MDSC in gastric cancer beating 615 mice (49.8％ ± 1.1％) was higher than the normal group (1.2％ ± 0.3％) (P ＜ 0.05).(3) Compared with normal group the MDSC expression in 1-MT + 5-FU group (18.5 ％ ± 0.5 ％) decreased significantly (P ＜ 0.05).Conclusions Combining 1-MT with 5-FU can reduce the number of MDSC in gastric cancer bearing 615 mice,improving tumor microenvironment.