Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus / 한국실험동물학회지
Laboratory Animal Research
;
: 365-368, 2011.
Article
in English
| WPRIM
| ID: wpr-45061
ABSTRACT
Antioxidative and aldose reductase (AR)-inhibitory effects of a fermentation filtrate of Rubus coreanus (FRC) were investigated using corneal/retinal homogenate and lens cytosol, respectively. Rat corneal/retinal homogenate was treated with 50 microM FeCl3 in the presence of FRC (3.2-100 microg/mL) for 30 min at 37degrees C, and thiobarbituric acid-reactive substances (TBARS) was quantified as a lipid peroxidation parameter. FRC markedly suppressed the TBARS production in a concentration-dependent manner, leading to 50% (IC50) and 100% (IC100) inhibitory concentrations of 20 and 95 microg/mL, respectively, which was similar to the effect of butylated hydroxyanisole. Activity of AR from rat lens was assayed in the presence of FRC (1-31.6 microg/mL) at 25degrees C using glyceraldehyde as a substrate. FRC inhibited lens AR by 50% (IC50) and 90% (IC90) at approximately 2 and 31.6 microg/mL, respectively, comparable to the effect of quercetin. The results indicate that ERC could be a promising candidate for the improvement of eye injury and visual dysfunction of dry eye and diabetic patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quercetin
/
Butylated Hydroxyanisole
/
Lipid Peroxidation
/
Eye Injuries
/
Aldehyde Reductase
/
Thiobarbituric Acid Reactive Substances
/
Cytosol
/
Eye
/
Fermentation
/
Glyceraldehyde
Limits:
Animals
/
Humans
Language:
English
Journal:
Laboratory Animal Research
Year:
2011
Type:
Article
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