Subcellular localization of the transmembrane inner ear (Tmie) protein in a stable Tmie-expressing cell line / 한국실험동물학회지
Laboratory Animal Research
;
: 339-342, 2011.
Article
in English
| WPRIM
| ID: wpr-45067
ABSTRACT
Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Line
/
Blotting, Western
/
Cytoplasm
/
Deafness
/
Hearing Loss
/
Ear, Inner
/
Membranes
Language:
English
Journal:
Laboratory Animal Research
Year:
2011
Type:
Article
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