Interaction of hypoxia inducible factor expression inhibition and P53 expression of patients with hepatic cellular cancer / 中国综合临床

ABSTRACT

Objective To investigate the correlation of hypoxia-inducible factors (HIF),P53 level and liver cancer infiltration in the microenvironment of the liver cancer pathological.Methods Flow cytometry was used to determine the P53 expression.Immunohistochemical method was applied to detect the expression of HIF characteristics,the correlation between Liver function (alanine amiotransferase (ALT),albumin (ALB),alpha fetoprotein(AFP))and HIF,P53.Results HIP positive rate in patients with encapsulated with incomplete,tumor diameter ≤ 5 cm and＞ 5 cm,tumor stage Ⅰ+Ⅱ and Ⅲ were 50.0％ (14/28) and 33.3％ (14/42),43.6 ％ (17/39)and 22.6％ (7/31),29.7％ (11/37)and 18.2％ (6/33)respectively.The HIF positive rate decrease in patients with capsular damage,tumor diameter increases (＞ 5 cm),Ⅲ patients,and differences between groups were statistically significant (P =0.040,0.030,0.030,respectively).P53 positive expression rate were 42.9％ (12/28) vs.83.3％ (35/42),51.3％ (20/39) vs.93.5％ (29/31),35.1％ (13/37) vs.69.7％ (23/33).The P53 positive expression in patients with capsular damage,tumor diameter increases (＞ 5 cm),Ⅲ patients with were relatively higher,the difference between groups was statistically significant (P =0.030,0.030,0.040,respectively).HIP positive rate in patient with past suffering from chronic hepatitis,cirrhosis and chronic AFP ＞ 35 ng/L,ALT ＞ 80 U/L were 36.2％ (21/58),27.9％ (17/61),27.1％ (14/51),28.2％ (11/39),lower than that of 58.3％ (7/12) in non-chronic hepatitis,non-cirrhotic 55.0％ (5/9),AFP ＜35 ng/L 58.0％ (11/19),ALT ＜ 80 U/L 51.6％ (16/31) of patients,the differences were statistically significant (P =0.046,0.047,0.037,0.045,respectively).P53 positive rate in patient with past sufering from chronic cirrhosis,AFP ＞ 35 ng/L,ALT ＞ 80 U/L,albumin ＜ 35 g/L were 82.0％ (50/61),74.5％ (38/51),69.2％ (27/39),73.7％ (42/57),respectively,higher than that of patient without chronic cirrhosis 44.4％ (4/9),or with AFP ＜ 35 ng/L 31.6％ (6/19),ALT ＜ 80 U/L 22.6％ (7/31),albumin ＞ 35 g/L 46.2％ (6/13).Tthe differences were statistically significant (P =0.037,0.040,0.032,0.041,respectively).Conclusion HIF and P53 might be the important factor in monitoring the development of hepatocellular carcinoma invasion.