Effects of erythropoietin on neural function and brain cell apoptosis in rats after cardiopulmonary resuscitation / 中华行为医学与脑科学杂志

ABSTRACT

Objective To study the effect of erythropoietin (EPO) on neural function and brain cell apoptosis in rats after cardiopulmonary resuscitation.Methods Adult male SD rats were randomly divided into control group and EPO group with 24 in each group.A rat model of asphyxial cardiac arrest and cardiopulmonary resuscitation was established.The neurological functions were assessed using neurological deficit score (NDS) 12 h and 24 h after cardiopulmonary resuscitation.The expressions of the apoptosis-inducing factor (AIF) and caspase-3 mRNA in cerebral cortex tissue were detected using reverse transcription-polymerase chain reaction (RT-PCR) at 0 h,12 h,and 24 h,respectively.Results Compared with the DNS scores in the control group (12 h:(60.00± 3.38) ;24 h:(54.50±2.56),respectively),12 h and 24 h NDS scores were (70.50±4.04) and (65.88±2.64) in EPO group after cardiopulmonary resuscitation,and the difference was statistically different (P＜0.01).The AIF mRNA expression levels of 12 h (1.31±0.26) and 24 h (1.87±0.17) after cardiopulmonary resuscitation in EPO group were obviously lower than those in the control group (12 h:(1.88 ± 0.18),24h:(2.71 ± 0.24),respectively),and the differences were statistically different (P＜0.01).The Caspas-3 mRNA expression levels of 12 h (1.49± 0.15) and 24 h (1.56±0.10) after cardiopuhmonary resuscitation in EPO group were obviously lower than those in the control group (12 h:(1.68± 0.10),24h:(1.84 ± 0.16),respectively),and the differences were statistically significant (P＜0.01).Conclusion EPO can reduce AIF and caspase-3 mRNA transcription,reduce apoptosis in cortical neurons caused by the cerebral ischemia and reperfusion injury after cardiopulmonary resuscitation,and therefore improve brain function.