Effect of propofol post-conditioning on synaptic transmission in neurons in CA 1 region during oxygen-glucose deprivation and restoration in hippocampi in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of propofol post-conditioning on synaptic transmission in neurons in CA1 region during oxygen-glucose deprivation and restoration (OGD/R) in hippocampi rats .Methods Hippocampi were isolated from male Wistar rats ,aged 15-20 days ,weighing 50-60 g ,and sliced at 350μm thick . The hippocampal slices were divided into 3 groups ( n=12 each ) using a random number tablecontrol group (group C) ,OGD/R group ,and propofol post-conditioning group (group P) .In group C ,the hippocampal slices were cultured in normal artificial cerebro-spinal fluid (nACSF ) for 7 min + 1 h . In OGD/R group , the hippocampal slices were incubated in glucose-free ACSF aerated with 95% N2 for 7 min followed by restoration of O2-glucose supply for 1 h .In group P ,the hippocampal slices were incubated in glucose-free ACSF aerated with 95% N2 for 7 min followed by incubation with normal ACSF containing propofol 1.2μg/ml for 1 h .The intensity and frequencies of spontaneous excitatory postsynaptic currents (sEPSC ) and spontaneous inhibitory postsynaptic current (sIPSC ) in neurons in CA1 region were recorded by whole-cell patch-clamp technique .Results Compared with group C ,the intensity and frequency of sEPSC were significantly increased ,while the intensity and frequency of sIPSC were decreased in OGD/R and P groups ( P<0.05) .Compared with group OGD/R ,the intensity and frequency of sEPSC were significantly decreased ,while the intensity and frequency of sIPSC were increased in group P ( P<0.05) .Conclusion The mechanism by which propofol post-conditioning attenuates OGD/R injury to hippocampi is related to inhibition of excitatory synaptic transmission and enhancement of inhibitory synaptic transmission in neurons in CA1 region .