Adhesion of enolase and Tuf from B.longum NCC2705 / 军事医学

ABSTRACT

Objective To demonstrate that enolase and Tuf are direct adhesions of Bifidobacterium longum( B.longum) NCC2705.Methods B.lougum NCC2705 was co-cultured with Caco-2.The change of adhesion was tested by microscopy after the purified proteins(GST-Eno and GST-Tuf) were added into the co-culture, and then statistical analysis was conduc-ted.Caco-2 cells were incubated with B.lougum NCC2705 and pathogenic bacteria (Shigella flexneri 2a or Salmonella). The level ofcytokine LDH and TNF-αfor supernatant was tested after the purified proteins ( GST-Eno and GST-Tuf) were added.Results The number of bacteria adherent to Caco-2 was reduced significantly ( P <0.05 ) after the purified proteins ( GST-Eno/GST-Tuf) were added .While Caco-2 was co-cultured with pathogens , the level of LDH and TNF-αwas significantly increased (P<0.05).When Caco-2 was incubated with B.lougum NCC2705 and pathogenic bacteria , the level of LDH and TNF-αwas significantly reduced (P<0.05), but the level of LDH and TNF-αincreased once more (P<0.05) after the purified protein (GST-Eno/GST-Tuf) was added.Conclusion B.longum NCC2705 can competitive-ly inhibit production of pathogenic bacteria in intestinal epithelial cell adhesion , while the enolization enzyme and Tuf proteins can competitively inhibit long bifidobacteria in intestinal epithelial cell adhesion effect .They are adhesion factors of bifidobacteria .