Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 104-108, 2015.
Article
in Korean
| WPRIM
| ID: wpr-45176
ABSTRACT
PURPOSE:
To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers.METHODS:
HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities.RESULTS:
The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p 0.05).CONCLUSIONS:
BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Trabecular Meshwork
/
Benzalkonium Compounds
/
Dexamethasone
/
Cell Survival
/
Mitomycin
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS