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Determination of the main phenolic in Clerodendranthus spicatus from different areas by colorimetric method / 国际药学研究杂志
Article in Zh | WPRIM | ID: wpr-452212
Responsible library: WPRO
ABSTRACT
Objective To choose an appropriate coloured system and develop a method for the determination of the main phenolic in Dai Medicine -Clerodendranthus spicatus from different areas. Methods The main phenolic in Clerodendranthus spicatus from 19 areas was determined at 500 nm wavelength, with rosmarinic acid as reference and sodium nitrite-aluminum nitrate-sodium hydroxide as colour-developing system. Results Rosmarinic acid presented good linear relationship (r2=0.9993) in the concentration range of 8.792-17.584μg/ml.The average recovery was 100.73%and RSD was 0.85%.The content of the main phenolic in Clerodendranthus spicatus ranged from 1.16 % to 3.49 %. Conclusion The method is appropriate for the determination of the main phenolic in Clerodendranthus spicatus. The main phenolic content in samples from Yunnan province is relatively high.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of International Pharmaceutical Research Year: 2014 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of International Pharmaceutical Research Year: 2014 Type: Article